Using the experimental data in the Excel spreadsheet posted on Blackboard, calculate the concentration of glucose in the original solution. Report the relative standard deviation.

 

Preparation of Glucose Working Standard Solution

 

Mass vial + glucose - 15.2874g

Mass empty vial - 14.5432g

Volume of Stock Standard - 0.2500 L

Volume of Stock Solution - 5.00 mL

Volume of Working Standard Solution - 250.0 mL

 

Spectrophotometric Measurements

Final Volume for Standard Solutions - 50.0 mL

 

test tube vol glucose added / mL absorbance @ 420nm

1 0.0 0.593

2 2.0 0.531

3 4.0 0.479

4 6.0 0.421

5 8.0 0.372

6 10.0 0.305

Unknown 1 5.0 0.411

Unknown 2 5.0 0.412

Unknown 3 5.0 0.410

 

Volume Stock Unknown - 5.00 mL

 

 

Correct Result

 

[glucose]_original = 76.1 mg L^-1

 

% RSD = 0.55

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