1.For each combination of numerical aperture and wavelength, calculate the resolution and determine if two points found at specified distances apart would be resolvable as discrete points.

Wavelength = 500 nm, NA = 0.8, Distance between points = 400 nm

Resolution?

Resolvable?

Wavelength = 600 nm, NA = 1.2, Distance between points = 253 nm

Resolution?

Resolvable?

 

2,When growing, many bacteria produce acid byproducts as a result of their metabolism. Too much acidity can inhibit bacterial growth. As such, media often includes some buffering capacity to limit the change in pH. Which components of the recipes for NA and GSA do you think help accomplish buffering of each medium?

 

3.Agar is especially useful for the cultivation of bacteria. One reason is that most bacteria are not able to utilize agar as a carbon source. Explain why this is so important when studying metabolic capabilities of bacteria, or attempting to perform an enrichment culture of a particular type of bacteria? Agar is also very useful in cultivating bacteria because of its melting and solidifying properties. Why is this? At what temperature does agar melt, and at what temperature does it solidify when cooling?

 

4,Ignoring that you probably wouldn't want to consume NA or GSA, do you think either of these media could support human growth if consumed? Explain fully.

 

Thank You, if any of my questions are not clear please inform me.

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