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Category > Biology Posted 19 Nov 2017 My Price 12.00

need help with my molecular bio homework

Hello, I need help with my molecular bio homework. 

Please find it attached

Thanks.

 

Bioinformatics question (see last page for vector):aHere are the forward and reverse primers for a hypothetical geneX.5’ – ATG GCA ATC CAA TCA ATA GGT CG – 3’5’ – TTA ACC CTT GAT GAT CGT TCT CC – 3’As per the map in page 2,modify the primers above in a way thatwill allow directional cloning of the gene Xto include the FLAGtag upstream and c-myc protein tag downstream of geneX.Afteryou are finished, the vector should express ONLY the one formof the labeled protein, specifically both tags flanking protein Xat the appropriate ends.You can use any of the enzymesexceptSal I and Xba I. You may modify the primer sequences by addingand removing (cross-out) the necessary nucleotides to satisfy theconditions of this task.b.Why can you NOT use Sma I only to clone in the gene?
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Status NEW Posted 19 Nov 2017 05:11 AM My Price 12.00

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