1.    List and explain each step in the Gram staining procedure. explain how or why Gram staining works and give an example of a Gram-positive and Gram-negative microorganism.

2.    Using a good compound light microscope with a resolving power of 0.3 µm, a 10X ocular lens, and a 100X oil immersion lens, would you be able to discern two objects separated by 3 µm? 0.3 µm? 300 nm? Why would or wouldn't a person be able to perform this with the presented microscope?

3.    Explain Why isn't the Gram stain used on acid-fast bacteria?

4.    Describe the pathway of light from its source to the eye, explaining what happens as it passes through the major parts of the microscope. Using a compound binocular light microscope, how can one obtain a total magnification of 2000X with a 100X objective?

 

Provide information regarding microbial size, shape, motility, and differentiation of the following preparations: spore stain, negative stain, simple stain, hanging drop slide, and Gram stain. Could the Gram stain be used to diagnose the flu? Why or why not?

 

complete each question1.List and explain each step in the Gram staining procedure. Furthermore, explain how or why Gram stainingworks and give an example of a Gram-positive and Gram-negative microorganism.2.Using a good compound light microscope with a resolving power of 0.3 µm, a 10X ocular lens, and a 100Xoil immersion lens, would you be able to discern two objects separated by 3 µm? 0.3 µm? 300 nm? Whywould or wouldn't you be able to do this with the presented microscope?3.Why isn't the Gram stain used on acid-fast bacteria? Explain your answer.4.Describe the pathway of light from its source to the eye, explaining what happens as it passes through themajor parts of the microscope. Using a compound binocular light microscope, how can one obtain a totalmagnification of 2000X with a 100X objective?Provide information regarding microbial size, shape, motility, and differentiation of the following preparations:spore stain, negative stain, simple stain, hanging drop slide, and Gram stain. Could the Gram stain be used todiagnose the flu? Why or why not?

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