1.    The isoelectric point, pI, is the pH at which the overall charge on the protein is zero. At a pH below pI, the protein will become positively charged overall.
At a pH above pI, the protein will become negatively charged overall.
(i)Â Â Â Â Â Â Â Â Â Â Â Â Â Explain what happens to BSA (pI=4.7) if a loading buffer of pH 7 is used, followed by an eluting buffer of pH 11.
Discuss the charges on BSA and DEAE at the two pH’s.
Include whether the protein binds or elutes.
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         (ii) Explain what happens to BSA (pI=4.7) if a loading buffer of pH 7 is used, followed by an eluting buffer of pH 3.5.
Give the charges on BSA and DEAE at the two pH’s.
Include whether the protein binds or elutes.
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(iii)Â Explain what happens to BSA (pI=4.7)Â if a loading buffer of pH 11 is used.
Give the charges on BSA and DEAE at this pH.
Include whether the protein binds or elutes.
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Posted 20 May 2017 03:05 AM
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