You are studying gene Q in yeast, which is an eukaryote but normally grows as a haploid. Protein Q is 50 kDa. The restriction map of the wild-type allele of this gene is shown below:
You are studying 4 mutant strains with mutations in this gene. You analyze wild-type and each of the mutants in the following ways:
i) Southern blot: cut genomic DNA isolated from each strain with EcoRI, run on gel, blot, and probe with radioactive 4.8 kbp BamHI fragment of gene Q.
ii) Northern blot: run RNA isolated from each strain on gel, blot, and probe with radioactive 4.8 kbp BamHI fragment of gene Q.
iii) Western blot: run protein isolated from each strain on gel, blot, and probe with radioactive anti-Q antibodies.
15
Draw the resulting three blots from the following strains (include molecular weight or size standards):
a) wild-type. b) a mutant with a deletion of the region from 0.8 kbp to 1.0 kbp that partially inactivates the promoter, resulting in 5-fold lower levels of transcription. Assume that the translation rate of Q mRNA molecules (protein molecules per mRNA molecule) is normal. c) a mutant with a point nonsense mutation at 2.5 kbp. d) a point missense mutant at 2.6 kbp that prevents EcoRI from cutting at that site. e) a deletion of the region from 2.0 kbp to 2.5 kbp.     
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Posted 26 Apr 2017 12:04 AM
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