Lab 6. Mitosis eScience ref #: Lab 11 Experiment 3: The Importance of Cell Cycle Control
Some environmental factors can cause genetic mutations which result in a lack of proper cell cycle control
(mitosis). When this happens, the possibility for uncontrolled cell growth occurs. In some instances, uncontrolled growth can lead to tumors, which are often associated with cancer, or other biological diseases. In this experiment, you will review some of the karyotypic differences which can be observed when comparing
normal, controlled cell growth and abnormal, uncontrolled cell growth. A karyotype is an image of the complete set of diploid chromosomes in a single cell. Materials
*Computer Access
*Internet Access *You Must Provide Procedure:
1. Begin by constructing a hypothesis to explain what differences you might observe when comparing the
karyotypes of human cells which experience normal cell cycle control versus cancerous cells (which experience abnormal, or a lack of, cell cycle control). Record your hypothesis in Post-Lab Question 1.
Note: Be sure to include what you expect to observe, and why you think you will observe these features. Think about what you know about cancerous cell growth to help construct this information
2. Go online to find some images of abnormal karyotypes, and normal karyotypes. The best results will come
from search terms such as “abnormal karyotype”, “HeLa cells”, “normal karyotype”, “abnormal chromosomes”, etc. Be sure to use dependable resources which have been peer-reviewed
3. Identify at least five abnormalities in the abnormal images. Then, list and draw each image in the Data
section at the end of this experiment. Do these abnormalities agree with your original hypothesis?
Hint: It may be helpful to count the number of chromosomes, count the number of pairs, compare the
sizes of homologous chromosomes, look for any missing or additional genetic markers/flags, etc. 180 Mitosis
Data
1. 2. 3. 4. 5. 181 Post-Lab Questions
1. Record your hypothesis from Step 1 in the Procedure section here. 2. What do your results indicate about cell cycle control? 3. Suppose a person developed a mutation in a somatic cell which diminishes the performance
of the body’s natural cell cycle control proteins. This mutation resulted in cancer, but was
effectively treated with a cocktail of cancer-fighting techniques. Is it possible for this person’s
future children to inherit this cancer-causing mutation? Be specific when you explain why or
why not. © eScience Labs, LLC 2014

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