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Teaching Since: | Apr 2017 |
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Questions Answered: | 12843 |
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MBA, Ph.D in Management
Harvard university
Feb-1997 - Aug-2003
Professor
Strayer University
Jan-2007 - Present
I isolate the entire genome of the bacteria E. coli. I then digest the genome with a restriction enzyme. I then ligate the digested fragments with the Ampicillin-resistance gene. Following digestion, I select for all the successfully ligated and circularized products, and then use these to transform E. coli. I plate the transformed cells on Ampicillin-containing media, and get some colonies.
a) What specific sequence from the E. coli genome MUST be present in plasmids that produce colonies on the Ampicillin-containing plates? Explain your answer.
b)Â MOST LIKELYÂ what might have been the purpose of this experiment? Explain.
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