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Elementary,Middle School,High School,College,University,PHD
Teaching Since: | May 2017 |
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Questions Answered: | 19234 |
Tutorials Posted: | 19224 |
MBA (IT), PHD
Kaplan University
Apr-2009 - Mar-2014
Professor
University of Santo Tomas
Aug-2006 - Present
Create a 2- to 3-page document in Microsoft Word for providing answers to questions in the following review sheets:
Support your responses with examples.
Cite any sources in APA format.
Exercise 4: Pure bacterial colonies
1. Â When an agar plate is inoculated, why is the loop sterilized after the initial inoculum is put on?
2. Â Distinguish between a pure culture and a mixed culture.
3. Â Define a bacterial colony. List four characteristics by which bacterial colonies may be distinguished.
4.Why should a Petri dish not be left open for any extended period?
5. Â Why does the streaking method you used to inoculate your plates result in isolated colonies?
Exercise 5: Pour plate and streaking technique to obtain pure cultures
1. Â Discuss the relative convenience of pour- and streak-plate techniques in culturing clinical specimens.
2. Â How do you decide which colonies should be picked from a plate culture of a mixed flora?
3. Â Why is it necessary to make pure subcultures of organisms grown from clinical specimens?
4. Â What kinds of clinical specimens may yield a mixed flora in bacterial cultures?
5. Â When more than one colony type appears in pure culture, what are the most likely sources of extraneous contamination?
Exercise 3: Primary media for isolation of microorganisms
1. Define a differential medium and discuss its purpose.
2. Define a selective medium and describe its uses.
3. Why is MacConkey agar selective as well as differential?
4. Why is blood agar useful as a primary isolation medium?
5. What is the major difference between Modified Thayer-Martin (MTM) and chocolate agar? When would you use MTM rather than chocolate agar?
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