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MBA, Ph.D in Management
Harvard university
Feb-1997 - Aug-2003
Professor
Strayer University
Jan-2007 - Present
You are monitoring an enzyme that converts a single substrate [S] into a product [P] in the presence of various concentrations of an unknown inhibitor [I]. Enter the data shown below into a graphing program (Excel or Deltagraph etc) and determine the following:
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1) (10 points) Diagnose the mechanism of inhibition. To accomplish this, plot a double reciprocal plot (best done as an overlay), slope and/or y-intercept replots, and any other replots that are necessary to diagnose the nature of the inhibition. State your findings for these plots. Note that the data are ideal and your fits should be very close to perfect (R2 should be >0.99 in most cases). (Hint, you should have at least 3 plots!).
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