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Category > Biology Posted 03 Jul 2017 My Price 7.00

Lab 6 – Gel Electrophoresis Extra Credit

Lab 6 – Gel Electrophoresis Extra Credit
10 points possible
Exercises:
1. Use a standardized DNA marker of known DNA fragment sizes to design a standard
curve.
2. Apply the standard curve to estimate fragment size of your PCR fragment product
Exercise 1:
(6pts)
1. Print out the picture of the gel from class with your PCR product results.
2. Using the lanes marked DNA Marker, measure from the wells the distance in mm each
DNA marker band traveled and note the distance traveled in the tables below.
3. Draw a standard curve using the semi-log graph provided below. Must include proper
axis labels and graph title. Standard DNA Marker
Fragment
size (bp) Migration distance
(mm) Standard DNA Marker
Fragment
size (bp) 10,000 1,000 8,000 900 6,000 800 5,000 700 4,000 600 3,000 500 2,500 400 2,000 300 1,500 200 1,200 100 Migration distance
(mm) Exercise 2.
(4 pts)
1. Using the correctly made standard curve above approximate the size of your PCR
product. Draw on the graph how you obtained the size to receive full credit. Approximate size of PCR product _______________________

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SuperTutor
(15)
Status NEW Posted 03 Jul 2017 02:07 AM My Price 7.00

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