Your Full Name: UMUC Biology 102/103
Lab 5: Meiosis
INSTRUCTIONS: On your own and without assistance, complete this Lab 5 Answer Sheet
electronically and submit it via the Assignments Folder by the date listed in the Course
Schedule (under Syllabus). Answer ALL questions in your own words. If a direct quote is needed, put the
words in quotation marks and cite the source. To conduct your laboratory exercises, use the Laboratory Manual located under
Course Content. Read the introduction and the directions for each exercise/experiment
carefully before completing the exercises/experiments and answering the questions. Save your Lab 5 Answer Sheet in the following format: LastName_Lab5 (e.g.,
Smith_Lab5). You should submit your document as a Word (.doc or .docx) file to the
assignment folder for best compatibility. Post a copy of the lab in Turnitin.com. There will be a 10 point deduction if this is
not done. If a photo is missing, there is a 10 point deduction. Please include a photo of the lab. Each photo must include a piece of paper with
the student’s name and the date the photo was taken.
Each question is worth 4 points
© eScience Labs, LLC 2014 Each set of meiosis photos/diagrams is worth 10 points.
The karyotype photos and table are worth 5 points each – 10 points total. © eScience Labs, LLC 2014 Pre-Lab Questions
1. Compare and contrast mitosis and meiosis. Describe at least 2 differences and 2 similarities. a. Similarity 1 b. Similarity 2 c. Difference 1 d. Difference 2 © eScience Labs, LLC 2014 2. List and describe 2 major events that occur during interphase.
a. Event 1 –
b. Event 2 - Experiment 1: Following Chromosomal DNA Movement
through Meiosis
Data Tables and Post-Lab Assessment
Take a picture of your results. Include a note with your name and date on a
piece of paper as well as the meiotic stage and include it in the picture. Trial 1 - Meiotic Division Beads Photo:
Prophase I - NO picture needed Metaphase I – NO picture needed Anaphase I - NO picture needed
© eScience Labs, LLC 2014 Telophase I - NO picture needed Prophase II - NO picture needed Metaphase II - NO picture needed Anaphase II - NO picture needed Telophase II - NO picture needed Cytokinesis - NO picture needed Trial 2 - Meiotic Division Beads Photo:
Prophase I Metaphase I © eScience Labs, LLC 2014 Anaphase I Telophase I Prophase II Metaphase II Anaphase II Telophase II Cytokinesis Post-Lab Questions
1. a. What is the ploidy of the DNA at the end of meiosis I? Haploid or Diploid?
© eScience Labs, LLC 2014 b. What about at the end of meiosis II? Haploid or Diploid? 2. How are meiosis I and meiosis II different (NOT mitosis and meiosis)? Describe at least 2
differences, including a major difference in one of the steps they both go through. a. Difference 1 – b. Difference 2 - © eScience Labs, LLC 2014 3. Why do you use non-sister chromatids to demonstrate crossing over? Explain what would
happen if sister chromatids were used instead. 4. What combinations of alleles could result from a crossover between AC and ac
chromosomes?
© eScience Labs, LLC 2014 5. In the model with beads, how many individual chromosomes were present when meiosis I
started? 6. a. How many nuclei are present at the end of meiosis II? b. How many individual chromosomes are in each? © eScience Labs, LLC 2014 7. Identify two ways that meiosis contributes to genetic recombination. Explain how each leads to genetic variation in germ cells. a. Process 1 – b. Process 2 - © eScience Labs, LLC 2014 8. Why is it necessary to reduce the number of chromosomes in gametes, but not in other
cells? What would happen if the chromosome number were not reduced in gametes? 9. Polar Bears have 74 chromosomes in every cell. Determine how many chromosomes you
would expect to find in the following: i. Sperm Cell: © eScience Labs, LLC 2014 ii. Egg Cell: iii. Daughter Cell from Mitosis: iv. Daughter Cell from Meiosis II: 10. Research and find a disease that is caused by chromosomal mutations. a. When does the mutation occur?
b. What chromosome is affected? © eScience Labs, LLC 2014 c. What are the consequences?
d. Describe the condition associated with the mutation.
e. Cite the resource used to help answer this question Experiment 2: The Importance of Cell Cycle Control
Data Tables and Post-Lab Assessment –
Post pics of Karyotypes Below (1 normal, 4 abnormal – refer to FAQ for an example)
1.
2.
3.
4.
5.
Karyotype # Normal or
Abnormal? Total Number of
Chromosomes Observations 1
2
3
4
5 Post-Lab Questions
1. Record your hypothesis from Step 1 in the Procedure section here before reviewing the
karyotypes.. © eScience Labs, LLC 2014 2. Review the karyotypes. What do your results indicate about cell cycle control (a cell’s ability
to regulate when and how mitosis happens)?
3. Suppose a person developed a mutation in a somatic cell which diminishes the
performance of the body’s natural cell cycle control proteins. This mutation resulted in
cancer, but was effectively treated with a cocktail of cancer-fighting techniques. Is it possible
for this person’s future children to inherit this cancer-causing mutation? Be specific when
you explain why or why not.
4. Why do cells which lack cell cycle control exhibit karyotypes which look physically different
than cells with normal cell cycle. 5. a. What are HeLa cells? Please describe what they are and their historical significance. b.Why are HeLa cells appropriate for this experiment about cell cycle control and
chromosomal abnormalities?? © eScience Labs, LLC 2014

 

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