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Category > Biology Posted 03 Jul 2017 My Price 7.00

You have purified a small protein.

How do i figure out this answer ?

 

You have purified a small protein. To determine its amino acid sequence you incubate the protein with trypsin and in a separate reaction you cleave the protein with cyanogen bromide.

Cleavage with trypsin yielded 5 peptides that were sequenced by Edman degradation as shown in the following.

1. Ser─Leu

2. Asp─Val─Arg

3. Val─Met─Glu─Lys

4. Ser─Gln─Met─His─Lys

5. Ile─Phe─Met─Leu─Cys─Arg

Cleavage with cyanogen bromide yielded 4 peptides that were sequenced by Edman degradation:

1. His─Lys─Ser─Leu

2. Asp─Val─Arg─Val─Met

3. Glu─Lys─Ile─Phe─Met

4. Leu─Cys─Arg─Ser─Gln─Met

What is the identity of the N-terminal amino acid in the intact protein? A. Ser

B. His

C. Asp

D. Glu

E. Ile

Answers

SuperTutor
(15)
Status NEW Posted 03 Jul 2017 06:07 AM My Price 7.00

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