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Category > Biology Posted 16 Dec 2017 My Price 12.00

Final Applied Lab Project

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Final Applied Lab Project (1 credit Lab Component)Addresses course outcomes 1-4:recognize and explain how the scientific method is used to solve problemsmake observations and discriminate between scientific and pseudoscientific explanationsweigh evidence and make decisions based on strengths and limitations of scientific knowledgeand the scientific methoduse knowledge of biological principles, the scientific method, and appropriate technologies to askrelevant questions, develop hypotheses, design and conduct experiments, interpret results, anddraw conclusionsThis is the culminating lab assessment in BIOL 103. It is designed to assess your ability to apply theprinciples of the scientific method.The Effect of low pH on Enzyme ActivityDesign an experiment in which you will test the effect of an acidic fluid on enzymatic activity. Recall:enzymes are proteins! To complete this project, it may be useful for you to first review the ScientificMethod Tutorial, information about pH and enzymes in the text book and course modules, Lab 1(Introduction to Science) and Lab 4 (Enzymes). As you review Lab 4, you will be reminded that there areseveral factors that impact enzymatic activity: pH, temperature, and amount of reagent. It is OK to use thesame enzyme/substrate/method as you did in lab 4 (but modify the treatment), or you can search on-lineto find a different enzyme/substrate/method for measuring enzyme activity for your project (include allreferences).As you design your experiment for this project, please remember that you are trying to examine howanacidic fluidwill modify the outcome of an enzymatic reaction. To successfully complete this project,you will need to identify the question(s) being asked in your experiment and the hypothesis that you aretesting. In your experimental design, you must clearly explain what you are doing. That means thatyou will need to identify the enzyme, the substrate, the acidic fluid used as treatment, the controltreatment and the method of measuring enzyme activity, as well as explain your experimental protocol.You must also thoroughly explain how the acidic fluid impacted enzyme activity based on the results fromyour own experiment as well as knowledge of enzymes and pH from the text book, modules, lab manualand potentially additional information sources.Hint:Keep in mind that the acid will change the environmental conditions of the experiment (for example,a low pH value could change the shape of the active site on the enzyme protein), without directlyparticipating in the reaction.Lab MaterialsYoumayneed all or some of the following, depending on your experimental design:Materials from your lab kit:pH paper

hydrogen peroxide solution (you can purchase this at a pharmacy if you have used up the bottlethat came with the lab kit)plastic beakers or cupsvinegaryeast (can be purchased at grocery store if more is needed)balloonsplastic bottlemarker for labeling of beakersCell phone/digital camera - pictures requiredYou may choose to use additional materials (different acidic fluids and/or different organisms and/ordifferent substrate if you chose an enzyme other than catalase).Outline:Include the following in your outline:Name of enzyme you will useName of organism (if applicable)The substrate and products in the chemical reactionMethod for measuring enzyme activityTreatment: acidic fluid(s), pH, length of exposure, how you will treat your samplesThe control(s) in the experimentHypothesisHow you will present your data (table and/or type of graph)Anything else you would like to get feedback on before you start your experimentWrite a lab report that includes the following:1. Title page: descriptive title, your name, course name, semester2. Introduction: general background information about enzymes and specific information about yourchosen enzyme, the question(s) that you are asking and a clear hypothesis for your experiment (20points).

3. Design an experiment. Provide a detailed description of the materials and methods used to conduct theexperiment. Identify control and experimental samples, as well as independent and dependent variables.Also include the methods used for data collection and analysis (20 points).4. Conduct the experiment and record your results. Take picture(s) of your results. What did youobserve? Present your data in table and/or graph format. Remember to label everything and include theunit of measure with all numbers (20 points)5. Use your knowledge of enzymes and pH to interpret and discuss your results. It may be necessary foryou to refer to the OLI course modules, lab manual and/or use additional information resources. Whateffect does the acidic treatment have on enzyme activity? Did you get the expected results? Explain. (20points)6. State a specific and accurate conclusion. Is your hypothesis supported by the results? Looking back,how could you have improved your experiment? (10 points)7. Include a list of references to all information sources used in APA format (5 points).Avoid PlagiarismIt is very important to write withyour own words. If you do copy one or two sentences directly (usesparingly), use quotation marks (") around the copied text, followed by an in-text reference. All informationsources need to be included in the reference list and as in-text references. Plagiarism will be reported tothe proper UMUC authority. Guidance on how to avoid plagiarism can be foundhere:https://www.umuc.edu/students/academic-integrity/ai-tutorial/academic-integrity-tutorial.htmlSubmissionSubmit your final applied lab project in the assignment folder by the due date specified in the courseschedule.

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