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Elementary,Middle School,High School,College,University,PHD
| Teaching Since: | May 2017 |
| Last Sign in: | 398 Weeks Ago, 6 Days Ago |
| Questions Answered: | 66690 |
| Tutorials Posted: | 66688 |
MCS,PHD
Argosy University/ Phoniex University/
Nov-2005 - Oct-2011
Professor
Phoniex University
Oct-2001 - Nov-2016
RNA transcripts can be translated in vitro using ribosomes, transfer RNAs, and other necessary constituents extracted from cells, but the first codon can be any sequence of three nucleotides (instead of AUG, which is used in vivo). A synthetic mRNA consists of the repeating tetranucleotide 5'-AUGC-3' and hence has the sequence 5'-AUGCAUGCAUGCAUGCAUGCAUGC . . . -3'. When this molecule is translated in vitro, the resulting polypeptide has the repeating sequence Met—His—Ala—Cys—Met—His—Ala—Cys . . . What does this result tell you about the number of nucleotides in a codon? Using the fact that the only methionine codon is 5'-AUG-3', deduce a codon for histidine (His), alanine (Ala), and cysteine (Cys). Would the result differ if the mRNA were translated from the 3' end to the 5' end instead of in the actual direction from the 5' and to the 3'end?
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