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MCS,PHD
Argosy University/ Phoniex University/
Nov-2005 - Oct-2011
Professor
Phoniex University
Oct-2001 - Nov-2016
From the following information determine the amino acid sequence of a peptide.
N-terminal Edman gives PTH-Alanine
C terminal carboxypeptidase treatment, no observable reaction
Trypsin cleavage gives three products
1. Arg
2. Peptide containing Ala, Lys
3. Peptide containing Asp, Met, Phe, Pro
Mild Chymotrypsin cleavage gives 2 peptides
1. Peptide containing Asp, Pro
2. Peptide containing Ala, Arg, Lys, Met, Phe
CNBr cleavage gives 2 peptides
1. Peptide containing Ala, Arg, Lys and homoserine
2. Peptide containing Asp, Phe, Pro
You must supply the answer as the 3-letter amino acid sequence from N-terminus to C-terminus in the form (you must use dashes, not spaces between the amino acids) for example;
Met-Thr-Glu-Trp
Hel-----------lo -----------Sir-----------/Ma-----------dam----------- Â----------- -----------Tha-----------nk -----------You----------- fo-----------r u-----------sin-----------g o-----------ur -----------web-----------sit-----------e a-----------nd -----------acq-----------uis-----------iti-----------on -----------of -----------my -----------pos-----------ted----------- so-----------lut-----------ion-----------. P-----------lea-----------se -----------pin-----------g m-----------e o-----------n c-----------hat----------- I -----------am -----------onl-----------ine----------- or----------- in-----------box----------- me----------- a -----------mes-----------sag-----------e I----------- wi-----------ll